paxchic.blogg.se

The data is now good to create a standard curve in Excel. Creating a linear standard curve in Microsoft Excel

You know if you have done this correctly as your corrected background sample should now read ‘ 0‘. I have done this below and called the column ‘ Absorbance (562 nm)‘: This will give background-corrected absorbance values. To do this, simply subtract the average absorbance values from the average absorbance value of the blank – so ‘ 0.0945‘. In this instance, before we can proceed we need to first correct the data for the background noise. What we have here is the average absorbances of each standard next to the corresponding known concentrations. To create the standard curve, I have measured the absorbance of 8 standards (25, 125, 250, 500, 750, 1000, 15 μg/mL total protein) and a blank sample (0 μg/mL total protein) for background corrections. The independent data is plotted on the x-axis, whereas the dependent data is plotted on the y-axis, on a scatter plot.įor this example, I will use data generated from the BCA protein assay kit to estimate total protein concentrations. the optical density readings of the samples). protein standard concentrations in a BCA assay), and the other is the dependent variable which refers to the measured values (e.g. One set of data must be the independent variable, which is the known values (e.g. To create a standard curve in Microsoft Excel, two data variables are required. I will use the BCA total protein assay standards as an example. In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown sample values.